Download MendeleyStructural and functional characterisation of a glycoside hydrolase family 3 beta-xylosidase from Thermotoga maritima
Gloster, T.M., Foltanyi, F.To be published.
8C7F
Crystal structure of beta-xylosidase mutant (D281N, E517Q) from Thermotoga maritima in complex with xylopentaose
- PDB DOI: https://doi.org/10.2210/pdb8C7F/pdb
- Classification: HYDROLASE
- Organism(s): Thermotoga maritima MSB8
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2023-01-15 Released: 2024-01-24
- Funding Organization(s): Other government
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.52 Å
- R-Value Free: 0.189
- R-Value Work: 0.164
Starting Model: experimental
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wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Xylosidase | 780 | Thermotoga maritima MSB8 | Mutation(s): 2 Gene Names: TM_0076 EC: 3.2.1.21 |  | |
UniProt | |||||
Find proteins for Q9WXT1 (Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)) Explore Q9WXT1 Go to UniProtKB: Q9WXT1 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q9WXT1 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Oligosaccharides
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MPD Query on MPD | E [auth A], F [auth B] | (4S)-2-METHYL-2,4-PENTANEDIOL C6 H14 O2 SVTBMSDMJJWYQN-YFKPBYRVSA-N |  | ||
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
| PRD_900117 Query on PRD_900117 | C [auth X] | 4beta-beta-xylotriose | Oligosaccharide / Metabolism |  | |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.52 Å
- R-Value Free: 0.189
- R-Value Work: 0.164
- Space Group: P 21 2 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 98.35 | α = 90 |
| b = 100.89 | β = 90 |
| c = 179.11 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| XDS | data reduction |
| XSCALE | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2024-01-24 Deposition Author(s): Gloster, T.M., Foltanyi, F.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Other government | -- |
Revision History (Full details and data files)
- Version 1.0: 2024-01-24
Type: Initial release
