Annotations: 2BTF

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2btfa1	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	Actin/HSP70	Actin	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
B [auth P]	d2btfp_	Alpha and beta proteins (a+b)	Profilin-like	Profilin (actin-binding protein)	Profilin (actin-binding protein)	Profilin (actin-binding protein)	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Actin-like ATPases	8092404	3000092	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Actin-like ATPases	8003798	3000092	SCOP2B (2022-06-29)
B [auth P]	SCOP2B Superfamily	Profilin (actin-binding protein)	8036091	3000452	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Actin_1st	e2btfA1	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_1st	ECOD (1.6)
A	Actin_2nd	e2btfA2	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_2nd	ECOD (1.6)
B [auth P]	Profilin	e2btfP1	A: a+b three layers	X: Profilin-like	H: profilin-like (From Topology)	T: profilin-like	F: Profilin	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.420.40	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
A	3.90.640.10	Alpha Beta	Alpha-Beta Complex	Actin	Chain A, domain 4	CATH (4.3.0)
B [auth P]	3.30.450.30	Alpha Beta	2-Layer Sandwich	Beta-Lactamase	Dynein light chain 2a, cytoplasmic	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00022	Actin (Actin)	Actin	-	Family
B [auth P]	PF00235	Profilin (Profilin)	Profilin	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	BETA-ACTIN	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding protein binding kinase binding enzyme binding protein kinase binding hydrolase activity catalytic activity structural constituent of postsynapse structural constituent of cytoskeleton structural molecule activity structural constituent of synapse structural constituent of postsynaptic actin cytoskeleton	cellular process cell motility multicellular organismal process axonogenesis cellular developmental process cell projection organization plasma membrane bounded cell projection morphogenesis cell development neuron projection development cell morphogenesis cellular component organization neurogenesis multicellular organism development cell projection morphogenesis cellular process cell motility multicellular organismal process axonogenesis cellular developmental process cell projection organization plasma membrane bounded cell projection morphogenesis cell development neuron projection development cell morphogenesis cellular component organization neurogenesis multicellular organism development cell projection morphogenesis neuron differentiation neuron projection morphogenesis cell differentiation anatomical structure development cellular component organization or biogenesis system development developmental process plasma membrane bounded cell projection organization nervous system development axon development cell morphogenesis involved in neuron differentiation generation of neurons anatomical structure morphogenesis neuron development	anchoring junction cell junction cellular anatomical entity focal adhesion cell-substrate junction protein-containing complex intracellular anatomical structure catalytic complex NuA4 histone acetyltransferase complex chromatin chromosome transferase complex H4/H2A histone acetyltransferase complex histone acetyltransferase complex anchoring junction cell junction cellular anatomical entity focal adhesion cell-substrate junction protein-containing complex intracellular anatomical structure catalytic complex NuA4 histone acetyltransferase complex chromatin chromosome transferase complex H4/H2A histone acetyltransferase complex histone acetyltransferase complex acetyltransferase complex H4 histone acetyltransferase complex intracellular organelle organelle intracellular non-membrane-bounded organelle protein acetyltransferase complex intracellular protein-containing complex non-membrane-bounded organelle membrane cytoskeleton actin cytoskeleton synapse cytoplasm cytosol polymeric cytoskeletal fiber actin filament supramolecular fiber supramolecular polymer supramolecular complex dense body cell periphery plasma membrane axon cell projection neuron projection plasma membrane bounded cell projection membrane-bounded organelle nucleus intracellular membrane-bounded organelle
B [auth P]	PROFILIN	cytoskeletal protein binding actin binding protein binding binding	positive regulation of organelle organization regulation of cytoskeleton organization regulation of cellular component organization regulation of cellular process positive regulation of cellular component organization regulation of actin filament-based process positive regulation of biological process regulation of actin filament bundle assembly cellular component organization cellular component biogenesis positive regulation of actin filament bundle assembly cellular component assembly actin filament bundle organization actin filament-based process positive regulation of organelle organization regulation of cytoskeleton organization regulation of cellular component organization regulation of cellular process positive regulation of cellular component organization regulation of actin filament-based process positive regulation of biological process regulation of actin filament bundle assembly cellular component organization cellular component biogenesis positive regulation of actin filament bundle assembly cellular component assembly actin filament bundle organization actin filament-based process cellular component organization or biogenesis positive regulation of cellular process regulation of biological process regulation of actin filament organization actin cytoskeleton organization positive regulation of cellular component biogenesis positive regulation of cytoskeleton organization biological regulation actin filament organization positive regulation of supramolecular fiber organization regulation of cellular component biogenesis regulation of organelle organization organelle organization actin filament bundle assembly regulation of actin cytoskeleton organization regulation of supramolecular fiber organization cellular process supramolecular fiber organization cytoskeleton organization regulation of cellular component size regulation of protein-containing complex assembly protein-containing complex assembly regulation of actin filament polymerization regulation of biological quality protein polymerization actin polymerization or depolymerization actin filament polymerization protein-containing complex organization regulation of actin polymerization or depolymerization regulation of protein polymerization regulation of actin filament length regulation of anatomical structure size	intracellular anatomical structure cytoplasm cellular anatomical entity intracellular organelle cytoskeleton organelle intracellular non-membrane-bounded organelle non-membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR020902	Actin/actin-like conserved site	Conserved Site
A	IPR004001	Actin, conserved site	Conserved Site
A	IPR043129	ATPase, nucleotide binding domain	Homologous Superfamily
A	IPR004000	Actin family	Family
B [auth P]	IPR036140	Profilin superfamily	Homologous Superfamily
B [auth P]	IPR027310	Profilin conserved site	Conserved Site
B [auth P]	IPR048278	Profilin	Family
B [auth P]	IPR005454	Profilin1/2/3, vertebrate	Family
B [auth P]	IPR005455	Profilin, eukaryotic type	Family

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A	ACE	RESID: AA0041 , AA0042 , AA0043 , AA0044 , AA0045 , AA0046 , AA0049 , AA0050 , AA0051 , AA0052 , AA0053 , AA0054 , AA0354 PSI-MOD : N-acetyl-L-alanine MOD:00050 , N-acetyl-L-aspartic acid MOD:00051 , N-acetyl-L-cysteine MOD:00052 , N-acetyl-S-archeol-cysteine MOD:00897 , N-acetyl-L-glutamic acid MOD:00053 , N-acetyl-L-glutamine MOD:00054 , N-acetylglycine MOD:00055 , N-acetyl-L-methionine MOD:00058 , N-acetyl-L-proline MOD:00059 , N-acetyl-L-serine MOD:00060 , N,O-diacetylated L-serine MOD:00648 , N-acetyl-L-threonine MOD:00061 , N-acetyl-L-tyrosine MOD:00062 , N-acetyl-L-valine MOD:00063 , N2-acetyl-L-arginine MOD:00359
A	HIC	Parent Component: HIS RESID: AA0041 , AA0042 , AA0043 , AA0044 , AA0045 , AA0046 , AA0049 , AA0050 , AA0051 , AA0052 , AA0053 , AA0054 , AA0354 , AA0317 PSI-MOD : N-acetyl-L-alanine MOD:00050 , N-acetyl-L-aspartic acid MOD:00051 , N-acetyl-L-cysteine MOD:00052 , N-acetyl-S-archeol-cysteine MOD:00897 , N-acetyl-L-glutamic acid MOD:00053 , N-acetyl-L-glutamine MOD:00054 , N-acetylglycine MOD:00055 , N-acetyl-L-methionine MOD:00058 , N-acetyl-L-proline MOD:00059 , N-acetyl-L-serine MOD:00060 , N,O-diacetylated L-serine MOD:00648 , N-acetyl-L-threonine MOD:00061 , N-acetyl-L-tyrosine MOD:00062 , N-acetyl-L-valine MOD:00063 , N2-acetyl-L-arginine MOD:00359 , 1'-methyl-L-histidine MOD:00322
B [auth P]	ACE	RESID: AA0041 , AA0042 , AA0043 , AA0044 , AA0045 , AA0046 , AA0049 , AA0050 , AA0051 , AA0052 , AA0053 , AA0054 , AA0354 , AA0317 , AA0041 , AA0042 , AA0043 , AA0044 , AA0045 , AA0046 , AA0049 , AA0050 , AA0051 , AA0052 , AA0053 , AA0054 , AA0354 PSI-MOD : N-acetyl-L-alanine MOD:00050 , N-acetyl-L-aspartic acid MOD:00051 , N-acetyl-L-cysteine MOD:00052 , N-acetyl-S-archeol-cysteine MOD:00897 , N-acetyl-L-glutamic acid MOD:00053 , N-acetyl-L-glutamine MOD:00054 , N-acetylglycine MOD:00055 , N-acetyl-L-methionine MOD:00058 , N-acetyl-L-proline MOD:00059 , N-acetyl-L-serine MOD:00060 , N,O-diacetylated L-serine MOD:00648 , N-acetyl-L-threonine MOD:00061 , N-acetyl-L-tyrosine MOD:00062 , N-acetyl-L-valine MOD:00063 , N2-acetyl-L-arginine MOD:00359 , 1'-methyl-L-histidine MOD:00322 , N-acetyl-L-alanine MOD:00050 , N-acetyl-L-aspartic acid MOD:00051 , N-acetyl-L-cysteine MOD:00052 , N-acetyl-S-archeol-cysteine MOD:00897 , N-acetyl-L-glutamic acid MOD:00053 , N-acetyl-L-glutamine MOD:00054 , N-acetylglycine MOD:00055 , N-acetyl-L-methionine MOD:00058 , N-acetyl-L-proline MOD:00059 , N-acetyl-L-serine MOD:00060 , N,O-diacetylated L-serine MOD:00648 , N-acetyl-L-threonine MOD:00061 , N-acetyl-L-tyrosine MOD:00062 , N-acetyl-L-valine MOD:00063 , N2-acetyl-L-arginine MOD:00359

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.