2ODE

Crystal structure of the heterodimeric complex of human RGS8 and activated Gi alpha 3

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d2odeb_	All alpha proteins	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d2oded_	All alpha proteins	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2 Family	Regulator of G-protein signaling, RGS	8062636	4001125	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Regulator of G-protein signaling, RGS	8062637	3000835	SCOP2 (2022-06-29)
D	SCOP2B Superfamily	Regulator of G-protein signaling, RGS	8062637	3000835	SCOP2B (2022-06-29)
A	SCOP2 Family	Galpha subunit-like	8056793	4004049	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Ras-like P-loop GTPases	8056794	3002022	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Galpha insert domain-like	8056795	3000669	SCOP2 (2022-06-29)
C	SCOP2B Superfamily	Ras-like P-loop GTPases	8056794	3002022	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Ras-like P-loop GTPases	8056794	3002022	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Galpha insert domain-like	8056795	3000669	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	RGS	e2odeB1	A: alpha bundles	X: Regulator of G-protein signaling, RGS (From Topology)	H: Regulator of G-protein signaling, RGS (From Topology)	T: Regulator of G-protein signaling, RGS	F: RGS	ECOD (1.6)
D	RGS	e2odeD1	A: alpha bundles	X: Regulator of G-protein signaling, RGS (From Topology)	H: Regulator of G-protein signaling, RGS (From Topology)	T: Regulator of G-protein signaling, RGS	F: RGS	ECOD (1.6)
A	G-alpha	e2odeA1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
A	Ras	e2odeA2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
C	G-alpha	e2odeC1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
C	Ras	e2odeC2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	1.10.196.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 1	CATH (4.3.0)
B	1.10.167.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 2	CATH (4.3.0)
D	1.10.196.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 1	CATH (4.3.0)
D	1.10.167.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 2	CATH (4.3.0)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
A	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)
C	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
C	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B, D	PF00615	Regulator of G protein signaling domain (RGS)	Regulator of G protein signaling domain	RGS family members are GTPase-activating proteins for heterotrimeric G-protein alpha-subunits.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B, D	Regulator of G-protein signaling 8	GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity molecular function regulator activity pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	cellular response to stimulus regulation of signaling regulation of biological process regulation of cellular process response to stimulus negative regulation of signaling signaling negative regulation of response to stimulus biological regulation negative regulation of biological process regulation of signal transduction negative regulation of signal transduction negative regulation of cell communication cell communication regulation of response to stimulus cellular response to stimulus regulation of signaling regulation of biological process regulation of cellular process response to stimulus negative regulation of signaling signaling negative regulation of response to stimulus biological regulation negative regulation of biological process regulation of signal transduction negative regulation of signal transduction negative regulation of cell communication cell communication regulation of response to stimulus negative regulation of cellular process regulation of cell communication cellular process signal transduction cellular response to nitrogen compound response to dopamine cellular response to dopamine response to monoamine regulation of dopamine receptor signaling pathway response to catecholamine cellular response to catecholamine stimulus regulation of G protein-coupled receptor signaling pathway response to organic cyclic compound G protein-coupled dopamine receptor signaling pathway cellular response to organic cyclic compound response to oxygen-containing compound response to nitrogen compound cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to monoamine stimulus response to chemical G protein-coupled receptor signaling pathway positive regulation of molecular function regulation of molecular function positive regulation of hydrolase activity regulation of catalytic activity regulation of hydrolase activity positive regulation of catalytic activity regulation of GTPase activity positive regulation of GTPase activity synaptic signaling cellular response to acetylcholine cell-cell signaling acetylcholine receptor signaling pathway postsynaptic signal transduction G protein-coupled acetylcholine receptor signaling pathway response to acetylcholine	somatodendritic compartment cell body perikaryon neuronal cell body cellular anatomical entity synapse cell junction cell periphery plasma membrane membrane neuronal cell body membrane cell body membrane cell projection dendrite somatodendritic compartment cell body perikaryon neuronal cell body cellular anatomical entity synapse cell junction cell periphery plasma membrane membrane neuronal cell body membrane cell body membrane cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection cytoplasmic side of plasma membrane cytoplasmic side of membrane side of membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle nucleus intracellular membrane-bounded organelle
A, C	Guanine nucleotide-binding protein G(k) subunit alpha	binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding GDP binding protein-containing complex binding G-protein beta/gamma-subunit complex binding protein binding signaling receptor binding G protein-coupled receptor binding cation binding metal ion binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	cellular process cell division cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction cellular response to nitrogen compound response to dopamine cellular process cell division cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction cellular response to nitrogen compound response to dopamine cellular response to dopamine response to monoamine response to catecholamine cellular response to catecholamine stimulus response to organic cyclic compound G protein-coupled dopamine receptor signaling pathway cellular response to organic cyclic compound response to oxygen-containing compound response to nitrogen compound cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to monoamine stimulus response to chemical regulation of catalytic activity negative regulation of molecular function negative regulation of catalytic activity negative regulation of adenylate cyclase activity regulation of molecular function regulation of lyase activity negative regulation of lyase activity regulation of adenylate cyclase activity negative regulation of cyclase activity regulation of cyclase activity catabolic process macroautophagy autophagy positive regulation of biological process positive regulation of cellular metabolic process regulation of autophagy cellular catabolic process positive regulation of metabolic process positive regulation of macroautophagy process utilizing autophagic mechanism positive regulation of cellular process positive regulation of autophagy cellular metabolic process regulation of cellular metabolic process regulation of catabolic process positive regulation of catabolic process regulation of macroautophagy regulation of cellular catabolic process positive regulation of cellular catabolic process metabolic process regulation of metabolic process adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway ribose phosphate metabolic process GTP metabolic process carbohydrate derivative metabolic process nucleobase-containing compound metabolic process purine nucleotide metabolic process ribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine ribonucleoside triphosphate metabolic process phosphate-containing compound metabolic process small molecule metabolic process phosphorus metabolic process ribonucleoside triphosphate metabolic process nucleoside phosphate metabolic process purine nucleoside triphosphate metabolic process primary metabolic process nucleotide metabolic process purine-containing compound metabolic process organophosphate metabolic process nucleoside triphosphate metabolic process purine ribonucleotide metabolic process	midbody cellular anatomical entity membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen midbody cellular anatomical entity membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen non-membrane-bounded organelle membrane protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space vesicle extracellular membrane-bounded organelle centrosome microtubule organizing center cytoskeleton microtubule cytoskeleton cytoplasm nucleoplasm vacuolar membrane lytic vacuole vacuole lytic vacuole membrane bounding membrane of organelle lysosomal membrane organelle membrane lysosome Golgi apparatus endomembrane system

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B, D	IPR016137	RGS domain	Domain
B, D	IPR036305	RGS domain superfamily	Homologous Superfamily
B, D	IPR034949	Regulator of G-protein signalling 8, RGS domain	Domain
B, D	IPR044926	RGS, subdomain 2	Homologous Superfamily
B, D	IPR024066	RGS, subdomain 1/3	Homologous Superfamily
A, C	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
A, C	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
A, C	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, C	IPR001408	G-protein alpha subunit, group I	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B, D	Pharos: P57771	astrocytic tumor facioscapulohumeral muscular dystrophy malignant ependymoma oligodendroglioma atypical teratoid rhabdoid tumor glioblastoma malignant glioma pilocytic astrocytoma diabetes mellitus large cell medulloblastoma medulloblastoma
A, C	Pharos: P08754	auriculocondylar syndrome dissociative amnesia auriculocondylar syndrome 1 large cell medulloblastoma malignant glioma oligodendroglioma pilocytic astrocytoma Albinism, Ocular psoriasis breast carcinoma intellectual disability lymphoplasmacytic lymphoma medulloblastoma osteosarcoma plasma cell myeloma auriculocondylar syndrome dissociative amnesia auriculocondylar syndrome 1 large cell medulloblastoma malignant glioma oligodendroglioma pilocytic astrocytoma Albinism, Ocular psoriasis breast carcinoma intellectual disability lymphoplasmacytic lymphoma medulloblastoma osteosarcoma plasma cell myeloma astrocytic tumor dermatomyositis Duchenne muscular dystrophy juvenile dermatomyositis malignant ependymoma myopathy ovarian cancer primitive neuroectodermal tumor amnestic disorder atypical teratoid rhabdoid tumor breast cancer diabetes mellitus glioblastoma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.