Annotations: 6HFR

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	D-HYD_C	e6hfrA1	A: beta sandwiches	X: Composite domain of metallo-dependent hydrolases (From Topology)	H: Composite domain of metallo-dependent hydrolases (From Topology)	T: Composite domain of metallo-dependent hydrolases	F: D-HYD_C	ECOD (1.6)
A	DHOase	e6hfrA2	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: DHOase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.20.20.140	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Metal-dependent hydrolases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01979	Amidohydrolase family (Amidohydro_1)	Amidohydrolase family	This family of enzymes are a a large metal dependent hydrolase superfamily [1]. The family includes Adenine deaminase EC:3.5.4.2 that hydrolyses adenine to form hypoxanthine and ammonia. Adenine deaminases reaction is important for adenine utilisatio ...	This family of enzymes are a a large metal dependent hydrolase superfamily [1]. The family includes Adenine deaminase EC:3.5.4.2 that hydrolyses adenine to form hypoxanthine and ammonia. Adenine deaminases reaction is important for adenine utilisation as a purine and also as a nitrogen source [2]. This family also includes dihydroorotase and N-acetylglucosamine-6-phosphate deacetylases, EC:3.5.1.25 These enzymes catalyse the reaction N-acetyl-D-glucosamine 6-phosphate + H2O <=> D-glucosamine 6-phosphate + acetate. This family includes the catalytic domain of urease alpha subunit [3]. Dihydroorotases (EC:3.5.2.3) are also included [4-5].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	CAD protein	dihydroorotase activity hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides catalytic activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds aspartate binding organic acid binding binding ion binding anion binding amino acid binding carboxylic acid binding small molecule binding carbon-nitrogen ligase activity, with glutamine as amido-N-donor dihydroorotase activity hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides catalytic activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds aspartate binding organic acid binding binding ion binding anion binding amino acid binding carboxylic acid binding small molecule binding carbon-nitrogen ligase activity, with glutamine as amido-N-donor ligase activity, forming carbon-nitrogen bonds carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity ligase activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups identical protein binding protein binding aspartate carbamoyltransferase activity carboxyl- or carbamoyltransferase activity transferase activity, transferring one-carbon groups hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides glutaminase activity cation binding transition metal ion binding zinc ion binding metal ion binding enzyme binding carbamoyl-phosphate synthase (ammonia) activity	developmental process heart development multicellular organism development multicellular organismal process anatomical structure development animal organ development circulatory system development system development response to ketone response to lipid response to stimulus response to chemical response to oxygen-containing compound response to testosterone developmental process heart development multicellular organism development multicellular organismal process anatomical structure development animal organ development circulatory system development system development response to ketone response to lipid response to stimulus response to chemical response to oxygen-containing compound response to testosterone response to organic cyclic compound response to nitrogen compound response to purine-containing compound response to caffeine response to alkaloid protein phosphorylation phosphorylation cellular metabolic process primary metabolic process macromolecule modification peptidyl-amino acid modification phosphate-containing compound metabolic process metabolic process cellular process peptidyl-threonine modification protein metabolic process macromolecule metabolic process protein modification process phosphorus metabolic process peptidyl-threonine phosphorylation response to endogenous stimulus response to alcohol response to cortisol response to glucocorticoid response to corticosteroid response to hormone response to steroid hormone protein autophosphorylation ribose phosphate metabolic process nucleotide biosynthetic process carbohydrate derivative metabolic process pyrimidine nucleotide metabolic process nucleobase-containing compound metabolic process ribonucleotide biosynthetic process ribonucleotide metabolic process pyrimidine ribonucleoside triphosphate biosynthetic process nucleobase-containing small molecule metabolic process small molecule metabolic process pyrimidine nucleoside triphosphate biosynthetic process pyrimidine-containing compound biosynthetic process ribose phosphate biosynthetic process pyrimidine ribonucleotide metabolic process ribonucleoside triphosphate metabolic process nucleoside phosphate metabolic process carbohydrate derivative biosynthetic process pyrimidine-containing compound metabolic process pyrimidine nucleoside triphosphate metabolic process nucleoside triphosphate biosynthetic process nucleotide metabolic process nucleobase-containing compound biosynthetic process ribonucleoside triphosphate biosynthetic process biosynthetic process organophosphate metabolic process organophosphate biosynthetic process nucleoside phosphate biosynthetic process pyrimidine ribonucleoside triphosphate metabolic process UTP biosynthetic process nucleoside triphosphate metabolic process UTP metabolic process pyrimidine nucleotide biosynthetic process pyrimidine ribonucleotide biosynthetic process regulation of body fluid levels secretion transport mammary gland development gland development biological regulation establishment of localization body fluid secretion localization lactation regulation of biological quality glutamine metabolic process oxoacid metabolic process carboxylic acid metabolic process amino acid metabolic process organic acid metabolic process alpha-amino acid metabolic process L-amino acid metabolic process glutamine family amino acid metabolic process proteinogenic amino acid metabolic process 'de novo' UMP biosynthetic process UMP biosynthetic process ribonucleoside monophosphate metabolic process UMP metabolic process nucleoside monophosphate metabolic process pyrimidine nucleoside monophosphate metabolic process pyrimidine ribonucleoside monophosphate metabolic process nucleoside monophosphate biosynthetic process pyrimidine ribonucleoside monophosphate biosynthetic process pyrimidine nucleoside monophosphate biosynthetic process ribonucleoside monophosphate biosynthetic process response to nutrient levels response to stress response to starvation hepaticobiliary system development liver development multi-multicellular organism process reproductive process female pregnancy multi-organism reproductive process pyrimidine nucleoside diphosphate metabolic process UDP metabolic process nucleoside diphosphate biosynthetic process pyrimidine ribonucleoside diphosphate metabolic process ribonucleoside diphosphate metabolic process pyrimidine ribonucleoside diphosphate biosynthetic process nucleoside diphosphate metabolic process UDP biosynthetic process pyrimidine nucleoside diphosphate biosynthetic process ribonucleoside diphosphate biosynthetic process cellular response to stimulus cellular response to chemical stimulus cellular response to xenobiotic stimulus xenobiotic metabolic process response to xenobiotic stimulus response to peptide hormone response to insulin small molecule biosynthetic process cellular biosynthetic process organic acid biosynthetic process carboxylic acid biosynthetic process non-proteinogenic amino acid metabolic process amino acid biosynthetic process citrulline biosynthetic process alpha-amino acid biosynthetic process citrulline metabolic process non-proteinogenic amino acid biosynthetic process nucleobase biosynthetic process pyrimidine nucleobase biosynthetic process pyrimidine nucleobase metabolic process nucleobase metabolic process 'de novo' pyrimidine nucleobase biosynthetic process animal organ regeneration regeneration cellular response to endogenous stimulus cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus response to epidermal growth factor response to growth factor response to amine	membrane cellular anatomical entity extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol membrane cellular anatomical entity extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol somatodendritic compartment cell body neuronal cell body nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle organelle lumen nuclear periphery nuclear lumen nuclear matrix axon synapse neuron projection axon terminus plasma membrane bounded cell projection presynapse cell projection distal axon terminal bouton cell junction neuron projection terminus protein-containing complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR029062	Class I glutamine amidotransferase-like	Homologous Superfamily
A	IPR006132	Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding	Domain
A	IPR002195	Dihydroorotase, conserved site	Conserved Site
A	IPR006680	Amidohydrolase-related	Domain
A	IPR036897	Carbamoyl-phosphate synthetase, large subunit oligomerisation domain superfamily	Homologous Superfamily
A	IPR006131	Aspartate/ornithine carbamoyltransferase, Asp/Orn-binding domain	Domain
A	IPR036901	Aspartate/ornithine carbamoyltransferase superfamily	Homologous Superfamily
A	IPR036914	Methylglyoxal synthase-like domain superfamily	Homologous Superfamily
A	IPR013815	ATP-grasp fold, subdomain 1	Homologous Superfamily
A	IPR011059	Metal-dependent hydrolase, composite domain superfamily	Homologous Superfamily
A	IPR005479	Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain	Domain
A	IPR006130	Aspartate/ornithine carbamoyltransferase	Family
A	IPR005480	Carbamoyl-phosphate synthetase, large subunit oligomerisation domain	Domain
A	IPR011607	Methylglyoxal synthase-like domain	Domain
A	IPR032466	Metal-dependent hydrolase	Homologous Superfamily
A	IPR002474	Carbamoyl-phosphate synthase small subunit, N-terminal domain	Domain
A	IPR017926	Glutamine amidotransferase	Domain
A	IPR036480	Carbamoyl-phosphate synthase small subunit, N-terminal domain superfamily	Homologous Superfamily
A	IPR002082	Aspartate carbamoyltransferase	Family
A	IPR005483	Carbamoyl-phosphate synthase large subunit, CPSase domain	Domain
A	IPR011761	ATP-grasp fold	Domain
A	IPR006274	Carbamoyl-phosphate synthase, small subunit	Family
A	IPR006275	Carbamoyl-phosphate synthase, large subunit	Family
A	IPR016185	Pre-ATP-grasp domain superfamily	Homologous Superfamily
A	IPR035686	Carbamoyl-phosphate synthase small subunit, GATase1 domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P27708	beta thalassemia developmental and epileptic encephalopathy, 50 sickle cell anemia inherited aplastic anemia congenital disorder of glycosylation hereditary spherocytosis acute erythroid leukemia ovarian cancer Cooley's anemia glioblastoma inborn errors of metabolism lung cancer medulloblastoma primitive neuroectodermal tumor astrocytic tumor beta thalassemia developmental and epileptic encephalopathy, 50 sickle cell anemia inherited aplastic anemia congenital disorder of glycosylation hereditary spherocytosis acute erythroid leukemia ovarian cancer Cooley's anemia glioblastoma inborn errors of metabolism lung cancer medulloblastoma primitive neuroectodermal tumor astrocytic tumor colorectal cancer hereditary persistence of fetal hemoglobin malignant glioma non-small cell lung carcinoma psoriasis thalassemia minor atypical teratoid rhabdoid tumor epilepsy large cell medulloblastoma malignant mesothelioma