8AF2

Human Sterol Carrier Protein with unnatural amino acid 2,2'-bipyridine alanine incorporated at position 111

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	SCP2-like	8003946	3000148	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	SCP2-like	8003946	3000148	SCOP2B (2022-06-29)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF02036	SCP-2 sterol transfer family (SCP2)	SCP-2 sterol transfer family	This domain is involved in binding sterols. It is found in the SCP2 protein Swiss:P22307, as well as the C terminus of Swiss:P51659 the enzyme estradiol 17 beta-dehydrogenase EC:1.1.1.62. The UNC-24 protein Swiss:Q17372 contains an SPFH domain Pfam:P ...	This domain is involved in binding sterols. It is found in the SCP2 protein Swiss:P22307, as well as the C terminus of Swiss:P51659 the enzyme estradiol 17 beta-dehydrogenase EC:1.1.1.62. The UNC-24 protein Swiss:Q17372 contains an SPFH domain Pfam:PF01145 [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Enoyl-CoA hydratase 2	oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors (3R)-hydroxyacyl-CoA dehydrogenase (NAD+) activity catalytic activity enoyl-CoA hydratase activity hydro-lyase activity 3-hydroxyacyl-CoA dehydratase activity lyase activity carbon-oxygen lyase activity 3-hydroxyacyl-CoA dehydrogenase activity steroid dehydrogenase activity steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor 17-beta-hydroxysteroid dehydrogenase (NAD+) activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors (3R)-hydroxyacyl-CoA dehydrogenase (NAD+) activity catalytic activity enoyl-CoA hydratase activity hydro-lyase activity 3-hydroxyacyl-CoA dehydratase activity lyase activity carbon-oxygen lyase activity 3-hydroxyacyl-CoA dehydrogenase activity steroid dehydrogenase activity steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor 17-beta-hydroxysteroid dehydrogenase (NAD+) activity 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity identical protein binding protein binding binding protein homodimerization activity protein dimerization activity isomerase activity estradiol 17-beta-dehydrogenase [NAD(P)+] activity	developmental process multicellular organismal process cellular developmental process ossification cell differentiation cellular process osteoblast differentiation nucleoside phosphate metabolic process very long-chain fatty-acyl-CoA metabolic process primary metabolic process purine-containing compound metabolic process fatty acid derivative metabolic process nucleobase-containing compound metabolic process acyl-CoA metabolic process developmental process multicellular organismal process cellular developmental process ossification cell differentiation cellular process osteoblast differentiation nucleoside phosphate metabolic process very long-chain fatty-acyl-CoA metabolic process primary metabolic process purine-containing compound metabolic process fatty acid derivative metabolic process nucleobase-containing compound metabolic process acyl-CoA metabolic process organophosphate metabolic process nucleobase-containing small molecule metabolic process phosphate-containing compound metabolic process metabolic process sulfur compound metabolic process small molecule metabolic process fatty-acyl-CoA metabolic process amide metabolic process thioester metabolic process phosphorus metabolic process lipid metabolic process regulation of hormone levels steroid metabolic process biological regulation androgen metabolic process regulation of biological quality hormone metabolic process fatty acid metabolic process lipid catabolic process catabolic process oxoacid metabolic process carboxylic acid metabolic process lipid modification organic acid catabolic process organic acid metabolic process fatty acid catabolic process cellular catabolic process monocarboxylic acid catabolic process fatty acid oxidation carboxylic acid catabolic process fatty acid beta-oxidation monocarboxylic acid metabolic process lipid oxidation small molecule catabolic process estrogen metabolic process very long-chain fatty acid metabolic process reproductive process sex differentiation cell development developmental process involved in reproduction epithelial cell differentiation Sertoli cell differentiation male gonad development male sex differentiation multicellular organism development development of primary male sexual characteristics development of primary sexual characteristics anatomical structure development reproductive structure development system development epithelium development gonad development reproductive system development Sertoli cell development animal organ development tissue development epithelial cell development medium-chain fatty-acyl-CoA metabolic process	cellular anatomical structure membrane intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle organelle peroxisome microbody intracellular membrane-bounded organelle microbody membrane bounding membrane of organelle peroxisomal membrane cellular anatomical structure membrane intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle organelle peroxisome microbody intracellular membrane-bounded organelle microbody membrane bounding membrane of organelle peroxisomal membrane organelle membrane peroxisomal matrix membrane-enclosed lumen intracellular organelle lumen organelle lumen microbody lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR051687	Peroxisomal Beta-Oxidation Enzymes	Family
A, B	IPR002347	Short-chain dehydrogenase/reductase SDR	Family
A, B	IPR020904	Short-chain dehydrogenase/reductase, conserved site	Conserved Site
A, B	IPR036527	SCP2 sterol-binding domain superfamily	Homologous Superfamily
A, B	IPR003033	SCP2 sterol-binding domain	Domain
A, B	IPR029069	HotDog domain superfamily	Homologous Superfamily
A, B	IPR002539	MaoC-like dehydratase domain	Domain
A, B	IPR054357	Peroxisomal multifunctional enzyme type 2-like, N-terminal domain	Domain
A, B	IPR036291	NAD(P)-binding domain superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: P51659	d-bifunctional protein deficiency Perrault syndrome Zellweger spectrum disorders peroxisomal disease West syndrome Perrault syndrome 1 Peroxisomal Dysfunction, Single polycystic ovary syndrome Spasmus nutans developmental and epileptic encephalopathy lymphoplasmacytic lymphoma ovarian cancer allergic rhinitis leukodystrophy pancreatic ductal adenocarcinoma d-bifunctional protein deficiency Perrault syndrome Zellweger spectrum disorders peroxisomal disease West syndrome Perrault syndrome 1 Peroxisomal Dysfunction, Single polycystic ovary syndrome Spasmus nutans developmental and epileptic encephalopathy lymphoplasmacytic lymphoma ovarian cancer allergic rhinitis leukodystrophy pancreatic ductal adenocarcinoma tuberculosis dermatomyositis intellectual disability lung cancer

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.