8QQZ
Cryo-EM structure of the light-driven sodium pump ErNaR in the pentameric form at pH 8.0
ELECTRON MICROSCOPY
Refinement
| RMS Deviations | |
|---|---|
| Key | Refinement Restraint Deviation |
| r_dihedral_angle_3_deg | 15.914 |
| r_long_range_B_refined | 9.285 |
| r_long_range_B_other | 9.284 |
| r_dihedral_angle_2_deg | 9.075 |
| r_scangle_other | 7.69 |
| r_dihedral_angle_1_deg | 5.288 |
| r_scbond_it | 4.624 |
| r_scbond_other | 4.624 |
| r_mcangle_it | 4.384 |
| r_mcangle_other | 4.384 |
| Sample |
|---|
| Light-driven sodium pump ErNaR |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 437017 |
| Reported Resolution (Å) | 2.63 |
| Resolution Method | FSC 0.143 CUT-OFF |
| Other Details | |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | C5 |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 | ||||
| Refinement Space | |||||
| Refinement Protocol | |||||
| Refinement Target | |||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | |||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | GATAN K3 (6k x 4k) | ||||||||
| Electron Dose (electrons/Å**2) | 50 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | |
| Temperature (Kelvin) | |
| Microscope Model | FEI TITAN KRIOS |
| Minimum Defocus (nm) | 500 |
| Maximum Defocus (nm) | 2000 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2.7 |
| Imaging Mode | OTHER |
| Specimen Holder Model | |
| Nominal Magnification | 105000 |
| Calibrated Magnification | |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| PARTICLE SELECTION | cryoSPARC | 4.0.2 |
| CTF CORRECTION | cryoSPARC | 4.0.2 |
| INITIAL EULER ASSIGNMENT | cryoSPARC | 4.0.2 |
| FINAL EULER ASSIGNMENT | cryoSPARC | 4.0.2 |
| CLASSIFICATION | cryoSPARC | 4.0.2 |
| RECONSTRUCTION | cryoSPARC | 4.0.2 |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||
