2ONJ

Structure of the multidrug ABC transporter Sav1866 from S. aureus in complex with AMP-PNP

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.40 Å
  • R-Value Free: 0.278 
  • R-Value Work: 0.254 

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Literature

Structure of the multidrug ABC transporter Sav1866 from Staphylococcus aureus in complex with AMP-PNP.

Dawson, R.J.P., Locher, K.P.

(2007) FEBS Lett 581: 935-938

  • DOI: https://doi.org/10.1016/j.febslet.2007.01.073
  • Primary Citation of Related Structures:  
    2ONJ

  • PubMed Abstract: 

    Staphylococcus aureus Sav1866 is a bacterial homolog of the human ABC transporter Mdr1 that causes multidrug resistance in cancer cells. We report the crystal structure of Sav1866 in complex with adenosine-5'-(beta,gamma-imido)triphosphate (AMP-PNP) at 3.4A resolution and compare it with the previously determined structure of Sav1866 with bound ADP. Besides differences in the ATP-binding sites, no significant conformational changes were observed. The results confirm that the ATP-bound state of multidrug ABC transporters is coupled to an outward-facing conformation of the transmembrane domains.

    • Organizational Affiliation: 

    Institute of Molecular Biology and Biophysics, ETH Zurich HPK D14.3, 8093 Zurich, Switzerland.


Macromolecules
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Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Multidrug export ATP-binding/permease protein SAV1866
A, B
578Staphylococcus aureusMutation(s): 0 
EC: 3.6.3 (PDB Primary Data), 7.6.2 (UniProt)
Membrane Entity: Yes 
UniProt
Find proteins for Q99T13 (Staphylococcus aureus (strain Mu50 / ATCC 700699))
Explore Q99T13 
Go to UniProtKB:  Q99T13
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ99T13
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.40 Å
  • R-Value Free: 0.278 
  • R-Value Work: 0.254 
  • Space Group: C 1 2 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 160.961α = 90
b = 104.447β = 98.23
c = 181.391γ = 90
Software Package:
Software NamePurpose
CNSrefinement
PDB_EXTRACTdata extraction

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2007-03-13
    Type: Initial release
  • Version 1.1: 2008-05-01
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2023-12-27
    Changes: Data collection, Database references, Derived calculations