Download MendeleyCrystal structure of aminoglycoside phosphotransferase APH(2'')-IVa ADP complex
Kaplan, E., Leban, N., Chaloin, L., Guichou, J.-F., Lionne, C.To be published.
4N57
Crystal structure of aminoglycoside phosphotransferase APH(2'')-IVa ADP complex
- PDB DOI: https://doi.org/10.2210/pdb4N57/pdb
- Classification: TRANSFERASE
- Organism(s): Enterococcus casseliflavus
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2013-10-09 Released: 2014-12-17
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.35 Å
- R-Value Free: 0.248
- R-Value Work: 0.182
- R-Value Observed: 0.185
Starting Model: experimental
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This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| APH(2'')-Id | 321 | Enterococcus casseliflavus | Mutation(s): 2 Gene Names: aph(2'')-Id |  | |
UniProt | |||||
Find proteins for O68183 (Enterococcus casseliflavus) Explore O68183 Go to UniProtKB: O68183 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | O68183 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ADP Query on ADP | E [auth A], H [auth B] | ADENOSINE-5'-DIPHOSPHATE C10 H15 N5 O10 P2 XTWYTFMLZFPYCI-KQYNXXCUSA-N |  | ||
| MG Query on MG | C [auth A], D [auth A], F [auth B], G [auth B] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.35 Å
- R-Value Free: 0.248
- R-Value Work: 0.182
- R-Value Observed: 0.185
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 76.051 | α = 90 |
| b = 65.802 | β = 92.43 |
| c = 76.731 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| AMoRE | phasing |
| REFMAC | refinement |
| MOSFLM | data reduction |
| SCALA | data scaling |
| DNA | data collection |
Entry History
Deposition Data
- Released Date: 2014-12-17 Deposition Author(s): Kaplan, E., Leban, N., Chaloin, L., Guichou, J.-F., Lionne, C.
Revision History (Full details and data files)
- Version 1.0: 2014-12-17
Type: Initial release - Version 1.1: 2019-07-17
Changes: Advisory, Data collection, Refinement description - Version 1.2: 2023-09-20
Changes: Advisory, Data collection, Database references, Derived calculations, Refinement description
