Download MendeleyStructural insights into protein splicing inhibition by platinum therapeutics as potential anti-microbials
Chan, H., Pearson, S., Green, C., Li, Z., Zhang, J., Lippard, S., Belfort, G., Shekhtman, A., Li, H.M., Belfort, M.To be published.
5I0A
RecA mini intein in complex with cisplatin
- PDB DOI: https://doi.org/10.2210/pdb5I0A/pdb
- Classification: SPLICING
- Organism(s): Mycobacterium tuberculosis
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2016-02-03 Released: 2016-09-21
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.50 Å
- R-Value Free: 0.193
- R-Value Work: 0.170
- R-Value Observed: 0.172
Starting Model: experimental
View more details
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Intein | 144 | Mycobacterium tuberculosis | Mutation(s): 0 |  | |
UniProt | |||||
Find proteins for P9WHJ3 (Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)) Explore P9WHJ3 Go to UniProtKB: P9WHJ3 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P9WHJ3 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| CPT Query on CPT | B [auth A], C [auth A] | Cisplatin Cl2 H6 N2 Pt LXZZYRPGZAFOLE-UHFFFAOYSA-L |  | ||
| TCE Query on TCE | D [auth A] | 3,3',3''-phosphanetriyltripropanoic acid C9 H15 O6 P PZBFGYYEXUXCOF-UHFFFAOYSA-N |  | ||
| SO4 Query on SO4 | E [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.50 Å
- R-Value Free: 0.193
- R-Value Work: 0.170
- R-Value Observed: 0.172
- Space Group: P 21 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 57.269 | α = 90 |
| b = 64.051 | β = 90 |
| c = 37.167 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| DENZO | data reduction |
| SCALEPACK | data scaling |
| PHASER | phasing |
| PHENIX | refinement |
Entry History & Funding Information
Deposition Data
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | GM39422 |
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | GM44844 |
Revision History (Full details and data files)
- Version 1.0: 2016-09-21
Type: Initial release - Version 1.1: 2017-09-20
Changes: Author supporting evidence, Derived calculations - Version 1.2: 2019-12-25
Changes: Author supporting evidence - Version 1.3: 2023-09-27
Changes: Data collection, Database references, Refinement description
