Download MendeleyStructure of EGFP(enhanced green fluorescent protein) mutant - L232H at 0.153 nm
Kachalova, G.S., Popov, A.P., Simanovskaya, A.A., Lipkin, A.V.To be published.
5N9O
EGFP(enhanced green fluorescent protein) mutant - L232H
- PDB DOI: https://doi.org/10.2210/pdb5N9O/pdb
- Classification: Calcium binding protein
- Organism(s): Aequorea victoria
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2017-02-26 Released: 2018-03-21
- Funding Organization(s): Russian Foundation for Basic Research
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.53 Å
- R-Value Free: 0.211
- R-Value Work: 0.167
- R-Value Observed: 0.169
Starting Model: experimental
View more details
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Green fluorescent protein | 237 | Aequorea victoria | Mutation(s): 1 Gene Names: GFP |  | |
UniProt | |||||
Find proteins for P42212 (Aequorea victoria) Explore P42212 Go to UniProtKB: P42212 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P42212 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| AE4 Query on AE4 | B [auth A], C [auth A] | 3,6,9,12,15-PENTAOXAHEPTADECAN-1-OL C12 H26 O6 NJRFAMBTWHGSDE-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| CRO Query on CRO | A | L-PEPTIDE LINKING | C15 H17 N3 O5 |  | THR, TYR, GLY |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.53 Å
- R-Value Free: 0.211
- R-Value Work: 0.167
- R-Value Observed: 0.169
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 84.595 | α = 90 |
| b = 67.931 | β = 118.66 |
| c = 47.266 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| XDS | data reduction |
| SCALA | data scaling |
| MOLREP | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2018-03-21 Deposition Author(s): Kachalova, G.S., Popov, A.P., Simanovskaya, A.A., Krukova, M.V., Lipkin, A.V.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Russian Foundation for Basic Research | Russian Federation | 13-04-12084 |
Revision History (Full details and data files)
- Version 1.0: 2018-03-21
Type: Initial release - Version 1.1: 2024-01-17
Changes: Data collection, Database references, Refinement description - Version 1.2: 2024-11-13
Changes: Structure summary
