Find PDB Structures with Protein Purification Tags

02/11

Protein tags are peptide sequences attached to proteins for various experimental reasons, particularly for purification.   Advanced Search can be used to search for entries with these protein purification tags.

To find these structures, select the Sequence Features > Sequence Motif option and enter an exact sequence or a sequence pattern expressed by regular expression syntax. Regular expressions can be used to define complex sequence patterns.

Entering ^HHHHHH will search for a 6 residue long N-terminal polyhistidine-tag (His6 tag), while a search for HHHHHH will find any sequence with a (His)6 pattern. This query looks at the sequence originally studied, and will find entries where an His6 tag appears completely, in part, or not at all in the coordinates.

More details about the sequence motif search are available.

Entry 4bnx contains a His6 tag

News Index

• 12/30 Happy Holidays

• 12/23 ADIT To Be Retired for Depositions from X-ray Crystallography

• 12/23 Congratulations to 2014 Poster Prize Winners

• 12/16 Contact Customer Services with Questions and Feedback

• 12/09 Save References to Mendeley and Endnote

• 12/09 Inclusion of Large Structures in the PDB Archive

• 12/02 World AIDS Day Symposium

• 12/02 Join the RCSB PDB Team

• 12/02 Art of Science on Display

• 11/25 Dr Frank Allen 1944-2014

• 11/25 ADIT To Be Retired for Depositions from X-ray Crystallography

• 11/18 RCSB PDB Software Shared on GitHub

• 11/11 RCSB PDB Reference in the Top 100

• 11/11 New Website Release

• 11/04 Join the Conversation

• 10/28 Membrane Query and Annotation

• 10/28 Web Services for Accessing PDB Data

• 10/21 Comparison Tool for Exploring Sequence and Structure Alignments

• 10/14 Upcoming Meeting: SACNAS

• 10/07 Fall Newsletter Published

• 09/30 Map Structures to Sequences with Protein Feature View

• 09/23 Paper Published: RCSB PDB Mobile

• 09/16 Exploring Structure Quality

• 09/09 Head Back to School with PDB-101

• 09/02 Take the PDB-101 Survey

• 09/02 Join the RCSB PDB Team

• 08/26 Poster Prize Awarded at ISMB

• 08/26 Announcement: Meeting on Hybrid Methods

• 08/19 Poster Prize Awarded at IUCr

• 08/12 New Website Release

• 08/05 wwPDB Events at IUCr (August 5-12)

• 07/29 Personalize the RCSB PDB

• 07/22 Explore PDB Structures by Browsing Annotations

• 07/15 RCSB PDB Leadership Transition

• 07/08 Summer Newsletter Published

• 07/01 Quick Tour of Search Results

• 06/24 Depositing Data? Use the wwPDB Deposition & Annotation System

• 06/17 HIV Video Challenge Awards

• 06/10 Poster Prize Awarded at ACA

• 06/10 Papers Published

• 06/03 Vote Now!

• 05/27 RCSB PDB Mobile for iOS and Android

• 05/20 Special Events in Albuquerque at ACA 2014

• 05/20 Art of Science Travels to Paris, France

• 05/13 PDB Reaches 100,000

• 05/13 Molecular Machinery Update

• 05/06 Video Challenge for High School Students

• 04/29 New Website Release

• 04/22 Visit the USA Science and Engineering Festival Expo on April 26 & 27

• 04/22 DNA Day 2014: April 25

• 04/15 Resources for High School Video Challenge

• 04/08 Spring Newsletter Published

• 04/01 Build a Model of GFP

• 04/01 Our Best Wishes to Phil

• 03/25 Learn How to Create Molecular Animations

• 03/25 The Art of Systems Biology and Nanoscience

• 03/18 Visit the San Diego Festival of Science and Engineering on March 22

• 03/11 Author Profiles: Timeline Display of a Researcher's Structures

• 03/11 Meet the RCSB PDB Team

• 03/04 Membrane Query and Annotation

• 02/25 Video Challenge for High School Students

• 02/18 Releasing Structures on Hold for Publication

• 02/11 Find PDB Structures with Protein Purification Tags

• 02/04 Tour of Ligand Deposition

• 01/28 Binding Site Visualization

• 01/21 Big Picture on Proteins

• 01/14 RapiData 2014

• 01/14 Teacher Workshop in Boulder, Colorado

• 01/07 Winter Newsletter Published